Eliminating polyphenol oxidase from Nicotiana benthamiana improves recombinant protein yield and purity and facilitates native-state protein studies
This study demonstrates that genome editing to eliminate polyphenol oxidases in *Nicotiana benthamiana* significantly reduces enzymatic browning and protein crosslinking, thereby enhancing the yield, purity, and native-state integrity of recombinant proteins produced via agroinfiltration.